Construct: shRNA TRCN0000431011

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182828.2-843s21c1
DNA Barcode:
CCACTTCATGATGTCGCTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GDF7 (151449)
Gene Description:
growth differentiation factor 7
Transcript:
RefSeq NM_182828.2 (NON-CURRENT)
Match location:
Position 843 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000431011)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTTCATGATGTCGCTTTA
Hairpin Sequence:
5'-CCGG-CCACTTCATGATGTCGCTTTA-CTCGAG-TAAAGCGACATCATGAAGTGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTTCATGATGTCGCTTTACTCGAGTAAAGCGACATCATGAAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTTCATGATGTCGCTTTACTCGAGTAAAGCGACATCATGAAGTGG-3'

Other clones with same target sequence:

  1. TRCN0000068154