Construct: shRNA TRCN0000431160

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_194464.2-3189s21c1
DNA Barcode:
ATGTATCACAACCGTTCTTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mrvi1 (17540)
Gene Description:
MRV integration site 1
Transcript:
RefSeq NM_194464.2 (NON-CURRENT)
Match location:
Position 3189 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000431160)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGTATCACAACCGTTCTTTC
Hairpin Sequence:
5'-CCGG-ATGTATCACAACCGTTCTTTC-CTCGAG-GAAAGAACGGTTGTGATACAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGTATCACAACCGTTCTTTCCTCGAGGAAAGAACGGTTGTGATACATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGTATCACAACCGTTCTTTCCTCGAGGAAAGAACGGTTGTGATACAT-3'

Other clones with same target sequence:

(none)