Construct: shRNA TRCN0000431172

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053232.1-474s21c1
DNA Barcode:
GGTCACTAAATCCTGTACACT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vmn1r29 (113859)
Gene Description:
vomeronasal 1 receptor 29
Transcript:
RefSeq NM_053232.1 (CURRENT)
Match location:
Position 474 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000431172)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTCACTAAATCCTGTACACT
Hairpin Sequence:
5'-CCGG-GGTCACTAAATCCTGTACACT-CTCGAG-AGTGTACAGGATTTAGTGACC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTCACTAAATCCTGTACACTCTCGAGAGTGTACAGGATTTAGTGACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTCACTAAATCCTGTACACTCTCGAGAGTGTACAGGATTTAGTGACC-3'

Other clones with same target sequence:

(none)