Construct: shRNA TRCN0000431193

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020706.2-3480s21c1
DNA Barcode:
CCGTAATGATGATAGAGATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCAF4 (57466)
Gene Description:
SR-related CTD associated factor 4
Transcript:
RefSeq NM_020706.2 (CURRENT)
Match location:
Position 3480 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000431193)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTAATGATGATAGAGATAA
Hairpin Sequence:
5'-CCGC-CCGTAATGATGATAGAGATAA-CTCGAG-TTATCTCTATCATCATTACGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTAATGATGATAGAGATAACTCGAGTTATCTCTATCATCATTACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTAATGATGATAGAGATAACTCGAGTTATCTCTATCATCATTACGG-3'

Other clones with same target sequence:

(none)