Construct: shRNA TRCN0000431208

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001083.3-691s21c1
DNA Barcode:
TGATATCTGCTGACCGCTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDE5A (8654)
Gene Description:
phosphodiesterase 5A
Transcript:
RefSeq NM_001083.3 (NON-CURRENT)
Match location:
Position 691 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000431208)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATATCTGCTGACCGCTATT
Hairpin Sequence:
5'-CCGG-TGATATCTGCTGACCGCTATT-CTCGAG-AATAGCGGTCAGCAGATATCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATATCTGCTGACCGCTATTCTCGAGAATAGCGGTCAGCAGATATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATATCTGCTGACCGCTATTCTCGAGAATAGCGGTCAGCAGATATCA-3'

Other clones with same target sequence:

(none)