Construct: shRNA TRCN0000431301

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028177.3-579s21c1
DNA Barcode:
GTGGCTGCTGACTGGAACTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ndufab1 (70316)
Gene Description:
NADH dehydrogenase (ubiquinone) 1, alpha/beta subcomplex, 1
Transcript:
RefSeq NM_028177.3 (CURRENT)
Match location:
Position 579 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000431301)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGCTGCTGACTGGAACTTA
Hairpin Sequence:
5'-CCGG-GTGGCTGCTGACTGGAACTTA-CTCGAG-TAAGTTCCAGTCAGCAGCCAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGCTGCTGACTGGAACTTACTCGAGTAAGTTCCAGTCAGCAGCCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGCTGCTGACTGGAACTTACTCGAGTAAGTTCCAGTCAGCAGCCAC-3'

Other clones with same target sequence:

(none)