Construct: shRNA TRCN0000431384

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178639.4-138s21c1
DNA Barcode:
GGCACTTCTTGGACATCATCG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sfxn5 (94282)
Gene Description:
sideroflexin 5
Transcript:
RefSeq NM_178639.4 (CURRENT)
Match location:
Position 138 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000431384)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCACTTCTTGGACATCATCG
Hairpin Sequence:
5'-CCGG-GGCACTTCTTGGACATCATCG-CTCGAG-CGATGATGTCCAAGAAGTGCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCACTTCTTGGACATCATCGCTCGAGCGATGATGTCCAAGAAGTGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCACTTCTTGGACATCATCGCTCGAGCGATGATGTCCAAGAAGTGCC-3'

Other clones with same target sequence:

(none)