Construct: shRNA TRCN0000431464

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000526.4-908s21c1
DNA Barcode:
ATTCTGAACGAGATGCGTGAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KRT14 (3861)
Gene Description:
keratin 14
Transcript:
RefSeq NM_000526.4 (NON-CURRENT)
Match location:
Position 908 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000431464)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTCTGAACGAGATGCGTGAC
Hairpin Sequence:
5'-CCGG-ATTCTGAACGAGATGCGTGAC-CTCGAG-GTCACGCATCTCGTTCAGAAT-TTTTTGGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTCTGAACGAGATGCGTGACCTCGAGGTCACGCATCTCGTTCAGAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTCTGAACGAGATGCGTGACCTCGAGGTCACGCATCTCGTTCAGAAT-3'

Other clones with same target sequence:

(none)