Construct: shRNA TRCN0000431489

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005668.4-1258s21c1
DNA Barcode:
AGCGGTCAAATATCATTATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ST8SIA4 (7903)
Gene Description:
ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4
Transcript:
RefSeq NM_005668.4 (NON-CURRENT)
Match location:
Position 1258 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000431489)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCGGTCAAATATCATTATTA
Hairpin Sequence:
5'-CCGG-AGCGGTCAAATATCATTATTA-CTCGAG-TAATAATGATATTTGACCGCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCGGTCAAATATCATTATTACTCGAGTAATAATGATATTTGACCGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCGGTCAAATATCATTATTACTCGAGTAATAATGATATTTGACCGCT-3'

Other clones with same target sequence:

(none)