Construct: shRNA TRCN0000431492

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000824.4-358s21c1
DNA Barcode:
TGCCAACTCCACTAGCAATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GLRB (2743)
Gene Description:
glycine receptor beta
Transcript:
RefSeq NM_000824.4 (NON-CURRENT)
Match location:
Position 358 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000431492)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCCAACTCCACTAGCAATAT
Hairpin Sequence:
5'-CCGG-TGCCAACTCCACTAGCAATAT-CTCGAG-ATATTGCTAGTGGAGTTGGCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCCAACTCCACTAGCAATATCTCGAGATATTGCTAGTGGAGTTGGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCCAACTCCACTAGCAATATCTCGAGATATTGCTAGTGGAGTTGGCA-3'

Other clones with same target sequence:

(none)