Construct: shRNA TRCN0000431502

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021827.4-1554s21c1
DNA Barcode:
GCTGCGCAAAGAGCGAAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCDC81 (60494)
Gene Description:
coiled-coil domain containing 81
Transcript:
RefSeq NM_021827.4 (CURRENT)
Match location:
Position 1554 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000431502)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCGCAAAGAGCGAAATTT
Hairpin Sequence:
5'-CCGG-GCTGCGCAAAGAGCGAAATTT-CTCGAG-AAATTTCGCTCTTTGCGCAGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCGCAAAGAGCGAAATTTCTCGAGAAATTTCGCTCTTTGCGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCGCAAAGAGCGAAATTTCTCGAGAAATTTCGCTCTTTGCGCAGC-3'

Other clones with same target sequence:

(none)