Construct: shRNA TRCN0000431529

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013305.4-2177s21c1
DNA Barcode:
TATATCGAAGACTTAGCTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ST8SIA5 (29906)
Gene Description:
ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 5
Transcript:
RefSeq NM_013305.4 (NON-CURRENT)
Match location:
Position 2177 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000431529)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATATCGAAGACTTAGCTATG
Hairpin Sequence:
5'-CCGG-TATATCGAAGACTTAGCTATG-CTCGAG-CATAGCTAAGTCTTCGATATA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATATCGAAGACTTAGCTATGCTCGAGCATAGCTAAGTCTTCGATATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATATCGAAGACTTAGCTATGCTCGAGCATAGCTAAGTCTTCGATATA-3'

Other clones with same target sequence:

(none)