Construct: shRNA TRCN0000431577

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_213602.2-190s21c1
DNA Barcode:
TTCTCCCGACAGGCTCATTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SIGLEC15 (284266)
Gene Description:
sialic acid binding Ig like lectin 15
Transcript:
RefSeq NM_213602.2 (NON-CURRENT)
Match location:
Position 190 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000431577)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCTCCCGACAGGCTCATTTG
Hairpin Sequence:
5'-CCGC-TTCTCCCGACAGGCTCATTTG-CTCGAG-CAAATGAGCCTGTCGGGAGAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCTCCCGACAGGCTCATTTGCTCGAGCAAATGAGCCTGTCGGGAGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCTCCCGACAGGCTCATTTGCTCGAGCAAATGAGCCTGTCGGGAGAA-3'

Other clones with same target sequence:

(none)