Construct: shRNA TRCN0000431613

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033272.2-636s21c1
DNA Barcode:
GAGAGGGTAAACCCAATATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNH7 (90134)
Gene Description:
potassium voltage-gated channel subfamily H member 7
Transcript:
RefSeq NM_033272.2 (NON-CURRENT)
Match location:
Position 636 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000431613)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAGGGTAAACCCAATATTA
Hairpin Sequence:
5'-CCGG-GAGAGGGTAAACCCAATATTA-CTCGAG-TAATATTGGGTTTACCCTCTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAGGGTAAACCCAATATTACTCGAGTAATATTGGGTTTACCCTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAGGGTAAACCCAATATTACTCGAGTAATATTGGGTTTACCCTCTC-3'

Other clones with same target sequence:

(none)