Construct: shRNA TRCN0000431622

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011770.4-2065s21c1
DNA Barcode:
ATGATAACTTGGCTTGTTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ikzf2 (22779)
Gene Description:
IKAROS family zinc finger 2
Transcript:
RefSeq NM_011770.4 (CURRENT)
Match location:
Position 2065 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000431622)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGATAACTTGGCTTGTTTAT
Hairpin Sequence:
5'-CCGG-ATGATAACTTGGCTTGTTTAT-CTCGAG-ATAAACAAGCCAAGTTATCAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGATAACTTGGCTTGTTTATCTCGAGATAAACAAGCCAAGTTATCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGATAACTTGGCTTGTTTATCTCGAGATAAACAAGCCAAGTTATCAT-3'

Other clones with same target sequence:

(none)