Construct: shRNA TRCN0000431625

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002505.4-1169s21c1
DNA Barcode:
GATCCAAACCAAGCCGATGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NFYA (4800)
Gene Description:
nuclear transcription factor Y subunit alpha
Transcript:
RefSeq NM_002505.4 (NON-CURRENT)
Match location:
Position 1169 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000431625)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCCAAACCAAGCCGATGAA
Hairpin Sequence:
5'-CCGG-GATCCAAACCAAGCCGATGAA-CTCGAG-TTCATCGGCTTGGTTTGGATC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCCAAACCAAGCCGATGAACTCGAGTTCATCGGCTTGGTTTGGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCCAAACCAAGCCGATGAACTCGAGTTCATCGGCTTGGTTTGGATC-3'

Other clones with same target sequence:

(none)