Construct: shRNA TRCN0000431658

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145413.4-1200s21c1
DNA Barcode:
TCTAAAGAATGGAGTACTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam20b (215015)
Gene Description:
family with sequence similarity 20, member B
Transcript:
RefSeq NM_145413.4 (CURRENT)
Match location:
Position 1200 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000431658)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTAAAGAATGGAGTACTAAA
Hairpin Sequence:
5'-CCGG-TCTAAAGAATGGAGTACTAAA-CTCGAG-TTTAGTACTCCATTCTTTAGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTAAAGAATGGAGTACTAAACTCGAGTTTAGTACTCCATTCTTTAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTAAAGAATGGAGTACTAAACTCGAGTTTAGTACTCCATTCTTTAGA-3'

Other clones with same target sequence:

(none)