Construct: shRNA TRCN0000431674

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013244.3-1867s21c1
DNA Barcode:
GGCTAATTATTAGGAGTATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MGAT4C (25834)
Gene Description:
MGAT4 family member C
Transcript:
RefSeq NM_013244.3 (NON-CURRENT)
Match location:
Position 1867 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000431674)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCTAATTATTAGGAGTATTA
Hairpin Sequence:
5'-CCGG-GGCTAATTATTAGGAGTATTA-CTCGAG-TAATACTCCTAATAATTAGCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCTAATTATTAGGAGTATTACTCGAGTAATACTCCTAATAATTAGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCTAATTATTAGGAGTATTACTCGAGTAATACTCCTAATAATTAGCC-3'

Other clones with same target sequence:

(none)