Construct: shRNA TRCN0000431712

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016642.2-2809s21c1
DNA Barcode:
GACTTTGATCCCAACACTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPTBN5 (51332)
Gene Description:
spectrin beta, non-erythrocytic 5
Transcript:
RefSeq NM_016642.2 (NON-CURRENT)
Match location:
Position 2809 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000431712)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTTTGATCCCAACACTATA
Hairpin Sequence:
5'-CCGG-GACTTTGATCCCAACACTATA-CTCGAG-TATAGTGTTGGGATCAAAGTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTTTGATCCCAACACTATACTCGAGTATAGTGTTGGGATCAAAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTTTGATCCCAACACTATACTCGAGTATAGTGTTGGGATCAAAGTC-3'

Other clones with same target sequence:

(none)