Construct: shRNA TRCN0000431727

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004822.2-259s21c1
DNA Barcode:
GCATCCCGGACTTTGTCAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NTN1 (9423)
Gene Description:
netrin 1
Transcript:
RefSeq NM_004822.2 (NON-CURRENT)
Match location:
Position 259 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000431727)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCCCGGACTTTGTCAATG
Hairpin Sequence:
5'-CCGG-GCATCCCGGACTTTGTCAATG-CTCGAG-CATTGACAAAGTCCGGGATGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCCCGGACTTTGTCAATGCTCGAGCATTGACAAAGTCCGGGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCCCGGACTTTGTCAATGCTCGAGCATTGACAAAGTCCGGGATGC-3'

Other clones with same target sequence:

(none)