Construct: shRNA TRCN0000431736

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011265.3-2890s21c1
DNA Barcode:
TTGGCAGCAGATGCATCAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rfx3 (19726)
Gene Description:
regulatory factor X, 3 (influences HLA class II expression)
Transcript:
RefSeq NM_011265.3 (CURRENT)
Match location:
Position 2890 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000431736)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGGCAGCAGATGCATCAAAT
Hairpin Sequence:
5'-CCGG-TTGGCAGCAGATGCATCAAAT-CTCGAG-ATTTGATGCATCTGCTGCCAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGGCAGCAGATGCATCAAATCTCGAGATTTGATGCATCTGCTGCCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGGCAGCAGATGCATCAAATCTCGAGATTTGATGCATCTGCTGCCAA-3'

Other clones with same target sequence:

(none)