Construct: shRNA TRCN0000431787

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022127.1-1713s21c1
DNA Barcode:
GTAAACGGTGTGCAGCAATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC28A3 (64078)
Gene Description:
solute carrier family 28 member 3
Transcript:
RefSeq NM_022127.1 (NON-CURRENT)
Match location:
Position 1713 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000431787)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAAACGGTGTGCAGCAATAT
Hairpin Sequence:
5'-CCGG-GTAAACGGTGTGCAGCAATAT-CTCGAG-ATATTGCTGCACACCGTTTAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAAACGGTGTGCAGCAATATCTCGAGATATTGCTGCACACCGTTTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAAACGGTGTGCAGCAATATCTCGAGATATTGCTGCACACCGTTTAC-3'

Other clones with same target sequence:

(none)