Construct: shRNA TRCN0000431817

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010252.4-1769s21c1
DNA Barcode:
TTACTGGGTTGGGTATCTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gabrg1 (14405)
Gene Description:
gamma-aminobutyric acid (GABA) A receptor, subunit gamma 1
Transcript:
RefSeq NM_010252.4 (CURRENT)
Match location:
Position 1769 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000431817)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTACTGGGTTGGGTATCTATA
Hairpin Sequence:
5'-CCGG-TTACTGGGTTGGGTATCTATA-CTCGAG-TATAGATACCCAACCCAGTAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTACTGGGTTGGGTATCTATACTCGAGTATAGATACCCAACCCAGTAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTACTGGGTTGGGTATCTATACTCGAGTATAGATACCCAACCCAGTAA-3'

Other clones with same target sequence:

(none)