Construct: shRNA TRCN0000431839

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019850.2-2554s21c1
DNA Barcode:
TTGCTCGCAAACTGGATAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NGEF (25791)
Gene Description:
neuronal guanine nucleotide exchange factor
Transcript:
RefSeq NM_019850.2 (NON-CURRENT)
Match location:
Position 2554 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000431839)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGCTCGCAAACTGGATAAAG
Hairpin Sequence:
5'-CCGG-TTGCTCGCAAACTGGATAAAG-CTCGAG-CTTTATCCAGTTTGCGAGCAA-TTTTTTTGAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGCTCGCAAACTGGATAAAGCTCGAGCTTTATCCAGTTTGCGAGCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGCTCGCAAACTGGATAAAGCTCGAGCTTTATCCAGTTTGCGAGCAA-3'

Other clones with same target sequence:

(none)