Construct: shRNA TRCN0000431857

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015480.1-682s21c1
DNA Barcode:
ATCAGCCAGTACAAGCTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NECTIN3 (25945)
Gene Description:
nectin cell adhesion molecule 3
Transcript:
RefSeq NM_015480.1 (NON-CURRENT)
Match location:
Position 682 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000431857)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCAGCCAGTACAAGCTATTT
Hairpin Sequence:
5'-CCGG-ATCAGCCAGTACAAGCTATTT-CTCGAG-AAATAGCTTGTACTGGCTGAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCAGCCAGTACAAGCTATTTCTCGAGAAATAGCTTGTACTGGCTGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCAGCCAGTACAAGCTATTTCTCGAGAAATAGCTTGTACTGGCTGAT-3'

Other clones with same target sequence:

(none)