Construct: shRNA TRCN0000431927

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153764.1-1401s21c1
DNA Barcode:
TATGAAGCAGTCAACAATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNJ1 (3758)
Gene Description:
potassium inwardly rectifying channel subfamily J member 1
Transcript:
RefSeq NM_153764.1 (NON-CURRENT)
Match location:
Position 1401 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000431927)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATGAAGCAGTCAACAATTTA
Hairpin Sequence:
5'-CCGG-TATGAAGCAGTCAACAATTTA-CTCGAG-TAAATTGTTGACTGCTTCATA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATGAAGCAGTCAACAATTTACTCGAGTAAATTGTTGACTGCTTCATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATGAAGCAGTCAACAATTTACTCGAGTAAATTGTTGACTGCTTCATA-3'

Other clones with same target sequence:

(none)