Construct: shRNA TRCN0000431953

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001039519.1-350s21c1
DNA Barcode:
CATACAGATTCTGCGATAATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gtf2a2 (235459)
Gene Description:
general transcription factor II A, 2
Transcript:
RefSeq NM_001039519.1 (NON-CURRENT)
Match location:
Position 350 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000431953)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATACAGATTCTGCGATAATG
Hairpin Sequence:
5'-CCGG-CATACAGATTCTGCGATAATG-CTCGAG-CATTATCGCAGAATCTGTATG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATACAGATTCTGCGATAATGCTCGAGCATTATCGCAGAATCTGTATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATACAGATTCTGCGATAATGCTCGAGCATTATCGCAGAATCTGTATG-3'

Other clones with same target sequence:

(none)