Construct: shRNA TRCN0000431962

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080672.3-2184s21c1
DNA Barcode:
TGATACCAACACTGAACATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PHACTR3 (116154)
Gene Description:
phosphatase and actin regulator 3
Transcript:
RefSeq NM_080672.3 (NON-CURRENT)
Match location:
Position 2184 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000431962)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATACCAACACTGAACATTC
Hairpin Sequence:
5'-CCGG-TGATACCAACACTGAACATTC-CTCGAG-GAATGTTCAGTGTTGGTATCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATACCAACACTGAACATTCCTCGAGGAATGTTCAGTGTTGGTATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATACCAACACTGAACATTCCTCGAGGAATGTTCAGTGTTGGTATCA-3'

Other clones with same target sequence:

(none)