Construct: shRNA TRCN0000431993

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010251.2-2288s21c1
DNA Barcode:
AGATATGGAGAGAGTTAATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gabra4 (14397)
Gene Description:
gamma-aminobutyric acid (GABA) A receptor, subunit alpha 4
Transcript:
RefSeq NM_010251.2 (CURRENT)
Match location:
Position 2288 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000431993)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGATATGGAGAGAGTTAATTA
Hairpin Sequence:
5'-CCGG-AGATATGGAGAGAGTTAATTA-CTCGAG-TAATTAACTCTCTCCATATCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGATATGGAGAGAGTTAATTACTCGAGTAATTAACTCTCTCCATATCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGATATGGAGAGAGTTAATTACTCGAGTAATTAACTCTCTCCATATCT-3'

Other clones with same target sequence:

(none)