Construct: shRNA TRCN0000432057

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015226.2-556s21c1
DNA Barcode:
GTCACGAGACCTCACTTTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CLEC16A (23274)
Gene Description:
C-type lectin domain containing 16A
Transcript:
RefSeq NM_015226.2 (NON-CURRENT)
Match location:
Position 556 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000432057)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCACGAGACCTCACTTTATT
Hairpin Sequence:
5'-CCGG-GTCACGAGACCTCACTTTATT-CTCGAG-AATAAAGTGAGGTCTCGTGAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCACGAGACCTCACTTTATTCTCGAGAATAAAGTGAGGTCTCGTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCACGAGACCTCACTTTATTCTCGAGAATAAAGTGAGGTCTCGTGAC-3'

Other clones with same target sequence:

(none)