Construct: shRNA TRCN0000432102

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152523.2-1003s21c1
DNA Barcode:
TCCTTAGCAGATGACAACAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCNYL1 (151195)
Gene Description:
cyclin Y like 1
Transcript:
RefSeq NM_152523.2 (CURRENT)
Match location:
Position 1003 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000432102)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCTTAGCAGATGACAACAAC
Hairpin Sequence:
5'-CCGG-TCCTTAGCAGATGACAACAAC-CTCGAG-GTTGTTGTCATCTGCTAAGGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCTTAGCAGATGACAACAACCTCGAGGTTGTTGTCATCTGCTAAGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCTTAGCAGATGACAACAACCTCGAGGTTGTTGTCATCTGCTAAGGA-3'

Other clones with same target sequence:

(none)