Construct: shRNA TRCN0000432170

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017737.4-1935s21c1
DNA Barcode:
TACTAACTTCATTAGCATTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FNBP1L (54874)
Gene Description:
formin binding protein 1 like
Transcript:
RefSeq NM_017737.4 (NON-CURRENT)
Match location:
Position 1935 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000432170)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACTAACTTCATTAGCATTTC
Hairpin Sequence:
5'-CCGG-TACTAACTTCATTAGCATTTC-CTCGAG-GAAATGCTAATGAAGTTAGTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACTAACTTCATTAGCATTTCCTCGAGGAAATGCTAATGAAGTTAGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACTAACTTCATTAGCATTTCCTCGAGGAAATGCTAATGAAGTTAGTA-3'

Other clones with same target sequence:

(none)