Construct: shRNA TRCN0000432176

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139281.2-1365s21c1
DNA Barcode:
GAGCTTGGGACATGGATTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WDR36 (134430)
Gene Description:
WD repeat domain 36
Transcript:
RefSeq NM_139281.2 (CURRENT)
Match location:
Position 1365 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000432176)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCTTGGGACATGGATTAAT
Hairpin Sequence:
5'-CCGG-GAGCTTGGGACATGGATTAAT-CTCGAG-ATTAATCCATGTCCCAAGCTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCTTGGGACATGGATTAATCTCGAGATTAATCCATGTCCCAAGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCTTGGGACATGGATTAATCTCGAGATTAATCCATGTCCCAAGCTC-3'

Other clones with same target sequence:

(none)