Construct: shRNA TRCN0000432198

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015009.1-1634s21c1
DNA Barcode:
ATGATGACAGGAACGACTTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDZRN3 (23024)
Gene Description:
PDZ domain containing ring finger 3
Transcript:
RefSeq NM_015009.1 (NON-CURRENT)
Match location:
Position 1634 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000432198)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGATGACAGGAACGACTTTC
Hairpin Sequence:
5'-CCGG-ATGATGACAGGAACGACTTTC-CTCGAG-GAAAGTCGTTCCTGTCATCAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGATGACAGGAACGACTTTCCTCGAGGAAAGTCGTTCCTGTCATCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGATGACAGGAACGACTTTCCTCGAGGAAAGTCGTTCCTGTCATCAT-3'

Other clones with same target sequence:

(none)