Construct: shRNA TRCN0000432252

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007086.2-2476s21c1
DNA Barcode:
GCTTCTCGCTCTCGGAAATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WDHD1 (11169)
Gene Description:
WD repeat and HMG-box DNA binding protein 1
Transcript:
RefSeq NM_007086.2 (NON-CURRENT)
Match location:
Position 2476 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000432252)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCTCGCTCTCGGAAATTA
Hairpin Sequence:
5'-CCGG-GCTTCTCGCTCTCGGAAATTA-CTCGAG-TAATTTCCGAGAGCGAGAAGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCTCGCTCTCGGAAATTACTCGAGTAATTTCCGAGAGCGAGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCTCGCTCTCGGAAATTACTCGAGTAATTTCCGAGAGCGAGAAGC-3'

Other clones with same target sequence:

(none)