Construct: shRNA TRCN0000432262
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_005364.4-746s21c1
- DNA Barcode:
- AGTGCATGCAGGTGATCTTTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAGEA8 (4107)
- Gene Description:
- MAGE family member A8
- Transcript:
- RefSeq NM_005364.4 (NON-CURRENT)
- Match location:
- Position 746 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000432262)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- AGTGCATGCAGGTGATCTTTG
- Hairpin Sequence:
-
5'-CCGG-AGTGCATGCAGGTGATCTTTG-CTCGAG-CAAAGATCACCTGCATGCACT-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGAGTGCATGCAGGTGATCTTTGCTCGAGCAAAGATCACCTGCATGCACTTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAAGTGCATGCAGGTGATCTTTGCTCGAGCAAAGATCACCTGCATGCACT-3'
Other clones with same target sequence:
(none)
