Construct: shRNA TRCN0000432293

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032341.3-788s21c1
DNA Barcode:
GCCAAGTAGTGATGCTTTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DDI2 (84301)
Gene Description:
DNA damage inducible 1 homolog 2
Transcript:
RefSeq NM_032341.3 (NON-CURRENT)
Match location:
Position 788 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000432293)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGTAGTGATGCTTTATA
Hairpin Sequence:
5'-CCGG-GCCAAGTAGTGATGCTTTATA-CTCGAG-TATAAAGCATCACTACTTGGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGTAGTGATGCTTTATACTCGAGTATAAAGCATCACTACTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGTAGTGATGCTTTATACTCGAGTATAAAGCATCACTACTTGGC-3'

Other clones with same target sequence:

(none)