Construct: shRNA TRCN0000432310

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004115.3-1158s21c1
DNA Barcode:
TTTCTAGTTAGACGCTGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FGF14 (2259)
Gene Description:
fibroblast growth factor 14
Transcript:
RefSeq NM_004115.3 (CURRENT)
Match location:
Position 1158 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000432310)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTTCTAGTTAGACGCTGTAAA
Hairpin Sequence:
5'-CCGG-TTTCTAGTTAGACGCTGTAAA-CTCGAG-TTTACAGCGTCTAACTAGAAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTTCTAGTTAGACGCTGTAAACTCGAGTTTACAGCGTCTAACTAGAAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTTCTAGTTAGACGCTGTAAACTCGAGTTTACAGCGTCTAACTAGAAA-3'

Other clones with same target sequence:

(none)