Construct: shRNA TRCN0000432315

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020663.3-1538s21c1
DNA Barcode:
CCATGTGTCTCACATTCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RHOJ (57381)
Gene Description:
ras homolog family member J
Transcript:
RefSeq NM_020663.3 (NON-CURRENT)
Match location:
Position 1538 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000432315)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGTGTCTCACATTCATTT
Hairpin Sequence:
5'-CCGG-CCATGTGTCTCACATTCATTT-CTCGAG-AAATGAATGTGAGACACATGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGTGTCTCACATTCATTTCTCGAGAAATGAATGTGAGACACATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGTGTCTCACATTCATTTCTCGAGAAATGAATGTGAGACACATGG-3'

Other clones with same target sequence:

(none)