Construct: shRNA TRCN0000432317

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172815.3-1769s21c1
DNA Barcode:
AGCATGGACTCAGCGTTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rspo2 (239405)
Gene Description:
R-spondin 2
Transcript:
RefSeq NM_172815.3 (CURRENT)
Match location:
Position 1769 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000432317)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCATGGACTCAGCGTTATTT
Hairpin Sequence:
5'-CCGG-AGCATGGACTCAGCGTTATTT-CTCGAG-AAATAACGCTGAGTCCATGCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCATGGACTCAGCGTTATTTCTCGAGAAATAACGCTGAGTCCATGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCATGGACTCAGCGTTATTTCTCGAGAAATAACGCTGAGTCCATGCT-3'

Other clones with same target sequence:

(none)