Construct: shRNA TRCN0000432332

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001009562.4-1739s21c1
DNA Barcode:
GCTTGAAGGTAGCCCTAATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLCO1B7 (338821)
Gene Description:
solute carrier organic anion transporter family member 1B7 (putative)
Transcript:
RefSeq NM_001009562.4 (CURRENT)
Match location:
Position 1739 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000432332)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGAAGGTAGCCCTAATAT
Hairpin Sequence:
5'-CCGG-GCTTGAAGGTAGCCCTAATAT-CTCGAG-ATATTAGGGCTACCTTCAAGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGAAGGTAGCCCTAATATCTCGAGATATTAGGGCTACCTTCAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGAAGGTAGCCCTAATATCTCGAGATATTAGGGCTACCTTCAAGC-3'

Other clones with same target sequence:

(none)