Construct: shRNA TRCN0000432357

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024841.4-581s21c1
DNA Barcode:
TGCAAAGCAACGAGCTCTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRR5L (79899)
Gene Description:
proline rich 5 like
Transcript:
RefSeq NM_024841.4 (CURRENT)
Match location:
Position 581 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000432357)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCAAAGCAACGAGCTCTATG
Hairpin Sequence:
5'-CCGG-TGCAAAGCAACGAGCTCTATG-CTCGAG-CATAGAGCTCGTTGCTTTGCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCAAAGCAACGAGCTCTATGCTCGAGCATAGAGCTCGTTGCTTTGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCAAAGCAACGAGCTCTATGCTCGAGCATAGAGCTCGTTGCTTTGCA-3'

Other clones with same target sequence:

(none)