Construct: shRNA TRCN0000432397

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003247.2-3986s21c1
DNA Barcode:
GAAACCAGTTTGGTGATATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
THBS2 (7058)
Gene Description:
thrombospondin 2
Transcript:
RefSeq NM_003247.2 (NON-CURRENT)
Match location:
Position 3986 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000432397)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAACCAGTTTGGTGATATAT
Hairpin Sequence:
5'-CCGG-GAAACCAGTTTGGTGATATAT-CTCGAG-ATATATCACCAAACTGGTTTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAACCAGTTTGGTGATATATCTCGAGATATATCACCAAACTGGTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAACCAGTTTGGTGATATATCTCGAGATATATCACCAAACTGGTTTC-3'

Other clones with same target sequence:

(none)