Construct: shRNA TRCN0000432420

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152906.4-931s21c1
DNA Barcode:
CTTCACTGAGCGTAGCATGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TANGO2 (128989)
Gene Description:
transport and golgi organization 2 homolog
Transcript:
RefSeq NM_152906.4 (NON-CURRENT)
Match location:
Position 931 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000432420)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCACTGAGCGTAGCATGAT
Hairpin Sequence:
5'-CCGG-CTTCACTGAGCGTAGCATGAT-CTCGAG-ATCATGCTACGCTCAGTGAAG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCACTGAGCGTAGCATGATCTCGAGATCATGCTACGCTCAGTGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCACTGAGCGTAGCATGATCTCGAGATCATGCTACGCTCAGTGAAG-3'

Other clones with same target sequence:

(none)