Construct: shRNA TRCN0000432467

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015077.2-2019s21c1
DNA Barcode:
AGAACATTGTGCCCATCATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SARM1 (23098)
Gene Description:
sterile alpha and TIR motif containing 1
Transcript:
RefSeq NM_015077.2 (NON-CURRENT)
Match location:
Position 2019 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000432467)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAACATTGTGCCCATCATTG
Hairpin Sequence:
5'-CCGC-AGAACATTGTGCCCATCATTG-CTCGAG-CAATGATGGGCACAATGTTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAACATTGTGCCCATCATTGCTCGAGCAATGATGGGCACAATGTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAACATTGTGCCCATCATTGCTCGAGCAATGATGGGCACAATGTTCT-3'

Other clones with same target sequence:

(none)