Construct: shRNA TRCN0000432471

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199015.3-275s21c1
DNA Barcode:
AGACTCTTGATCTACGTAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Phf11d (219132)
Gene Description:
PHD finger protein 11D
Transcript:
RefSeq NM_199015.3 (NON-CURRENT)
Match location:
Position 275 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000432471)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGACTCTTGATCTACGTAATA
Hairpin Sequence:
5'-CCGG-AGACTCTTGATCTACGTAATA-CTCGAG-TATTACGTAGATCAAGAGTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGACTCTTGATCTACGTAATACTCGAGTATTACGTAGATCAAGAGTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGACTCTTGATCTACGTAATACTCGAGTATTACGTAGATCAAGAGTCT-3'

Other clones with same target sequence:

(none)