Construct: shRNA TRCN0000432472

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009631.3-1374s21c1
DNA Barcode:
TTCGCATGTGGTATCAGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adora3 (11542)
Gene Description:
adenosine A3 receptor
Transcript:
RefSeq NM_009631.3 (NON-CURRENT)
Match location:
Position 1374 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000432472)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCGCATGTGGTATCAGTAAA
Hairpin Sequence:
5'-CCGG-TTCGCATGTGGTATCAGTAAA-CTCGAG-TTTACTGATACCACATGCGAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCGCATGTGGTATCAGTAAACTCGAGTTTACTGATACCACATGCGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCGCATGTGGTATCAGTAAACTCGAGTTTACTGATACCACATGCGAA-3'

Other clones with same target sequence:

(none)