Construct: shRNA TRCN0000432539

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014606.1-1127s21c1
DNA Barcode:
TGTGGAGCAAGAGGTCAATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HERC3 (8916)
Gene Description:
HECT and RLD domain containing E3 ubiquitin protein ligase 3
Transcript:
RefSeq NM_014606.1 (NON-CURRENT)
Match location:
Position 1127 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000432539)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTGGAGCAAGAGGTCAATTA
Hairpin Sequence:
5'-CCGG-TGTGGAGCAAGAGGTCAATTA-CTCGAG-TAATTGACCTCTTGCTCCACA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTGGAGCAAGAGGTCAATTACTCGAGTAATTGACCTCTTGCTCCACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTGGAGCAAGAGGTCAATTACTCGAGTAATTGACCTCTTGCTCCACA-3'

Other clones with same target sequence:

(none)