Construct: shRNA TRCN0000432575

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004621.5-1299s21c1
DNA Barcode:
GATCCAGTCATGACGGCTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRPC6 (7225)
Gene Description:
transient receptor potential cation channel subfamily C member 6
Transcript:
RefSeq NM_004621.5 (NON-CURRENT)
Match location:
Position 1299 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000432575)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCCAGTCATGACGGCTTTA
Hairpin Sequence:
5'-CCGG-GATCCAGTCATGACGGCTTTA-CTCGAG-TAAAGCCGTCATGACTGGATC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCCAGTCATGACGGCTTTACTCGAGTAAAGCCGTCATGACTGGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCCAGTCATGACGGCTTTACTCGAGTAAAGCCGTCATGACTGGATC-3'

Other clones with same target sequence:

(none)