Construct: shRNA TRCN0000432582

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001005209.1-1463s21c1
DNA Barcode:
CCAGTGCGGGTATAGCCATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMEM198 (130612)
Gene Description:
transmembrane protein 198
Transcript:
RefSeq NM_001005209.1 (NON-CURRENT)
Match location:
Position 1463 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000432582)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTGCGGGTATAGCCATAT
Hairpin Sequence:
5'-CCGG-CCAGTGCGGGTATAGCCATAT-CTCGAG-ATATGGCTATACCCGCACTGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTGCGGGTATAGCCATATCTCGAGATATGGCTATACCCGCACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTGCGGGTATAGCCATATCTCGAGATATGGCTATACCCGCACTGG-3'

Other clones with same target sequence:

(none)